Nico is one of the heroes that appears in Alvin and the Chipmunks 2: Trouble in Rio de Janeiro alongside, Blu, Jewel, Rafael, and Nico. He along with Rafael, Blu, Jewel, and Pedro would later turned into horses by a furious Alvin Seville to make Simon, Theodore, Brittany, Jeanette, and Eleanor to pursues Marcel, Armando, and Tipa in order to prevent them from escaping with the ransom French and the captive birds. When the spell breaks at dawn, he was eventually turned back to his normal form.